Low-temperature FTIR spectroscopy of pharaonis phoborhodopsin
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چکیده
منابع مشابه
Low temperature FTIR spectroscopy and hydrogen bonding in cytosine polycrystals.
The FTIR spectra of both the pure NH and isotopically substituted ND (<10% and >90% D) polycrystalline cytosine were recorded in the range 400-4000 cm(-1) as a function of temperature (10-300 K). For the first time, uncoupled NH(D) stretching mode bands of amine and imine groups were observed in the spectra of isotopically diluted cytosine at low temperatures. These bands correspond to the thre...
متن کاملRole of Asp193 in chromophore-protein interaction of pharaonis phoborhodopsin (sensory rhodopsin II).
Pharaonis phoborhodopsin (ppR; also pharaonis sensory rhodopsin II, psRII) is a receptor of the negative phototaxis of Natronobacterium pharaonis. By spectroscopic titration of D193N and D193E mutants, the pK(a) of the Schiff base was evaluated. Asp193 corresponds to Glu204 of bacteriorhodopsin (bR). The pK(a) of the Schiff base (SBH(+)) of D193N was approximately 10.1-10.0 (at XH(+)) and appro...
متن کاملSelective Reaction of Hydroxylamine with Chromophore during Photocycle of pharaonis Phoborhodopsin by
Phoborhodopsin (pR; also called sensory rhodopsin II, sRII) is a receptor of negative phototaxis of Halobacterium salinarum, and pharaonis phoborhodopsin (ppR; also pharaonis sensory rhodopsin II, psRII) is a corresponding protein of Natronobacterium pharaonis. These receptors contain retinal as a chromophore which bind to a lysine residue via Schiff base. This Schiff base can be cleaved with h...
متن کاملProtein-Protein Interaction Changes in an Archaeal Light-Signal Transduction
Negative phototaxis in Natronomonas pharaonis is initiated by transient interaction changes between photoreceptor and transducer. pharaonis phoborhodopsin (ppR; also called pharaonis sensory rhodopsin II, psR-II) and the cognate transducer protein, pHtrII, form a tight 2 : 2 complex in the unphotolyzed state, and the interaction is somehow altered during the photocycle of ppR. We have studied t...
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In FT-ICLAS, the aim is to improve the absorption detection sensitivity. The experiment combines IntraCavity Laser Absorption Spectroscopy (ICLAS) with FTIR spectroscopy. ICLAS is a well known instrumental technique in which a sample is inserted in a cell placed inside a broadband, multimode laser cavity. The laser is operating in pulsed mode and intracavity absorption rises as the laser wave b...
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ژورنال
عنوان ژورنال: Seibutsu Butsuri
سال: 2001
ISSN: 0582-4052,1347-4219
DOI: 10.2142/biophys.41.s63_3